Materials

The following materials are available for the Elucidator system:

Integrative Proteomic-Genomic Kinetic Analysis of HIV Infection

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Applications of the Rosetta Elucidator® System in Drug Discovery and Development

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Rosetta Elucidator® System Brochure

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Support

If you are a Elucidator system customer, go to the Support area to access Elucidator system support materials:

- Documentation
- Software updates
- Known issues
- User forum

For an overview of available types of customer support, visit the Elucidator system configurations page.

Rosetta Elucidator System

What's New | Features | More Information | Evaluate

The Rosetta Elucidator® Protein Expression Data Management and Analysis System is a comprehensive solution for organizations involved in protein expression analysis.

From Data to Results.

Modern proteomics technologies and data collection workflows for LC/MS experiments present researchers with a wealth of data. The process for managing these rich and complex data sets requires a substantial investment of time and resources from researchers who wish to derive meaningful conclusions from their data.

The Rosetta Elucidator® system is a comprehensive software solution that empowers you to save valuable time, and solve the data management and analysis challenges posed by complex LC/MS experiments.

Tailored to meet the needs of proteomics research, the Elucidator system provides you with powerful data management capabilities, a highly customizable environment, an easy-to-use workspace, and dedicated customer support. The Elucidator system supports multiple workflows for the analysis of proteins, including label-free LC/MS, shotgun, and SILAC™ . Each workflow can be used with data from multidimensional separations.

What's New

To review the list of new enhancements for the latest version of the Elucidator system, download our PDF.

Features

The following features are described below:

Data Management

  • Built on Rosetta Biosoftware’s stable platform for the management of expression data.
  • Scales to meet the infrastructure needs from small-scale to the largest proteomics projects. 
  • Store and manage large volumes of mass spectrometry data.
  • Spend less time loading and formatting data with the Elucidator system's auto-importing tools.
  • Manage raw data, processed data, and associated protein annotations in one software solution.
  • Retrieve and view data at multiple summarization levels (e.g., feature (peak), isotope group, charge group, peptide, and protein-level data).
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Support for Multiple Workflows and Instruments

  • Supports label-free LC/MS, shotgun (Figure 1), and SILAC workflows. Each workflow can be used with data from multidimensional separations.
  • Integration with an ever expanding list of instruments from several vendors. Please visit What's New for a current list of instrument and workflows that we support.


Figure 1: Differential LC/MS and shotgun label-free data analysis workflows in the Elucidator system.
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Peak Detection

  • The PeakTeller™ algorithm in the Elucidator system provides sensitive peak detection, and accurate peak extraction and quantitation from complex mass spectrometry data.
  • Novel image processing steps of the PeakTeller™ algorithm generates processed, or aligned, data from label-free LC/MS experiments based on your experimental parameters.
  • Correct for run-to-run retention time variations by accurately aligning data from the differential LC/MS workflow across runs via the PeakTeller algorithm.
  • Use built-in statistical methods to maximize detection of differentially-expressed peaks and minimize false positives—even when few experimental replicates are available.
  • Take advantage of the automated de-isotoping and charge deconvolution of peptides to analyze data at multiple summarization levels.
  • Visualize raw and aligned data using the Peak Viewer (Figure 2).


Figure 2: Four different views of differential expression data provided by the Peak Viewer. The differential expression of a feature is displayed before alignment (top left) and after alignment (top right). Isotope group and charge state for pre-aligned, or raw, data are displayed (bottom left), and a bar chart summarizes peak intensity and p-values for aligned data (bottom right).
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Peptide and Protein Identification

  • Seamless integration with database search engines and open source tools built into the Elucidator system help you to efficiently identify proteins.
  • Create DTA files, submit to search engines, and import results for streamlined peptide and protein identification.
  • Integrate with Sequest®, MASCOT®, and X! Tandem search engines.
  • Manage peptide and protein database search results.
  • Verify correct peptide/protein assignments for all features with PeptideProphet™ and ProteinProphet®.
  • View search results, the theoretical spectrum, and other information related to individual features with the Verification Viewer (Figure 3).


Figure 3: The theoretical spectrum for Mascot search results displayed in the Verification Viewer.
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Experimental Definition

  • Analyze simple and complex experimental designs effectively that contain multiple factors and levels. (Figure 4)
  • Control the processing of raw data files to create processed data types based on the parameters of your experiment. 


Figure 4: The Elucidator System experiment definition: A sample experiment.
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Analysis Tools

  • Workflow interface for tracking the progress of each task in your end-to-end analysis (Figure 5).
  • Parametric and non-parametric significance tests, such as ANOVA, Wilcoxon, and t-Tests.
  • Data reduction methods (Multidimensional Scaling and Principal Component Analysis)
  • Supervised learning methods (classifiers).
  • Hierarchical and partitioning clustering methods, trend analysis.
  • Plots and interactive MS data viewers.
  • Visual scripts for data analysis for automating frequently-used workflows.
  • Ability to incorporate publicly available statistical tools or your own custom algorithms written in the R programming language for statistical computing.


Figure 5: Workflow interface for tracking steps and tasks in your analysis.
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Services and Support

  • Rosetta Professional Services can help you customize and integrate the Elucidator system with your current protein expression research efforts and existing bioinformatics tools.
  • Rosetta Technical Account Managers (TAMs) are trained scientists for routine or advanced support.
  • Application Scientists experienced in the mass spectrometry, proteomics, and informatics fields provide training which can be custom-tailored for your workflows.
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More information

For information about purchasing the Elucidator system, please contact our Sales Team.

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